Security is always about a balance between security and usability. One thing to look at is policy. What does current policy state? Policy should dictate what is and is not allowed. Check out the SANS policy institute for some free templates. You also need to consider if there is an acceptable use policy (AUP). With those in place you might want to start by denying access to objectionable websites. You should also block incoming traffic from areas of the world in which you do not do business.
You might also want to block IP's from known questionable addresses. Dshield.org has a suspicious networks block list you can check out. On internal computers and servers you need to make sure you have anti-virus installed, anti-spyware, and anti-malware installed.
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